Journal: Scientific Reports

Article Title: A simple label-free method reveals bacterial growth dynamics and antibiotic action in real-time

doi: 10.1038/s41598-022-22671-6

Figure Lengend Snippet: Growing cultures of K. pneumoniae, P. mirabilis, E. coli, E. faecium, S. saprophyticus and S. aureus, were diluted in medium at 37 °C to achieve concentrations between 10 1 and 10 7 cfu mL −1 and incubated in SLIC for up to 4 h. Time to positivity (TTP) was defined as the point when the mean value for scattering (mV) and 95% confidence intervals were greater than, and remained above, 0 mV (X- axis). n = 3.

Article Snippet: Two K. pneumoniae strains (ATCC BAA 1705 & 1706) were prepared from glycerol stock immediately before the experiment began.

Techniques: Incubation

Journal: Scientific Reports

Article Title: A simple label-free method reveals bacterial growth dynamics and antibiotic action in real-time

doi: 10.1038/s41598-022-22671-6

Figure Lengend Snippet: K. pneumoniae ATCC BAA-1705 and BAA-1706 were cultivated in paralell in SLIC. The output mV data from SLIC v7 over 600 min is plotted in ( A ) with the data for the first 120 min of the experiment is shown in ( B ) (one measurement per second with one in 480 points included in the graph). Points represent the mean of three independent measurements and error bars are the standard error of the mean. All data were fitted to the Gompertz and Broken stick equations (Eqs. and , respectively). The fitting results are illustrated in ( C,D ). Note from Table that the Broken Stick gave better fittings than the Gompertz equation, as determined by lower AIC values (see Table ).

Article Snippet: Two K. pneumoniae strains (ATCC BAA 1705 & 1706) were prepared from glycerol stock immediately before the experiment began.

Techniques:

Journal: Scientific Reports

Article Title: A simple label-free method reveals bacterial growth dynamics and antibiotic action in real-time

doi: 10.1038/s41598-022-22671-6

Figure Lengend Snippet: Values of lag phase ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\lambda$$\end{document} λ ), growth rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ ), asymptote (A) and AIC when fitted to the modified Gompertz equation (Eq. 1 ), and lag phase ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\lambda$$\end{document} λ ), growth rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ ), \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${c}_{0}$$\end{document} c 0 and AIC when fitted to the Broken Stick equation (Eq. 2 ) for Klebsiella pneumoniae ATCC BAA-1705 and BAA-1706.

Article Snippet: Two K. pneumoniae strains (ATCC BAA 1705 & 1706) were prepared from glycerol stock immediately before the experiment began.

Techniques: Modification

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Clinical carbapenemase-producing and -nonproducing Enterobacteriaceae strains analysed in this study.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: A) The spectrum obtained for the pure meropenem (arrived with ice) showed the peaks corresponding to the intact drug (384 ±1 Da) and to its sodium salts (406 ±1 Da; 428 ±1 Da); the spectrum obtained for the carbapenemase-nonproducing reference strain ( K . pneumoniae ATCC BAA-1706) showed the same peaks as the pure meropenem, while the 2 carbapenemase-producing reference strains ( K . pneumoniae ATCC BAA-1705, bla KPC positive, and K . pneumoniae ATCC BAA-2146, bla NDM positive) gave the peaks corresponding to the meropenem degradation (358 ±1 Da, the decarboxylated product) and to its sodium salts (380 ±1 Da; 402 ±1 Da; 424 ±1 Da). B) Example of spectra obtained for clinical samples tested with pure meropenem arrived with ice: a carbapenemase-nonproducing strain and two carbapenemase-producing strains, one of which ( K . pneumoniae No. 1135 strain) showing the peaks corresponding to both the pure meropenem and the degradation products of the drug.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Results of Meropenem Hydrolysis Assay (MHA) in case of intact or degraded drug in 1127 Enterobacteriaceae strains included in the panels I and II.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques: Hydrolysis Assay

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Results of Meropenem Hydrolysis Assay (MHA) in case of intact or degraded drug in 58 Enterobacteriaceae strains included in the panel III.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques: Hydrolysis Assay